Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine.

نویسندگان

  • Laura E McNamara
  • Terje Sjöström
  • R M Dominic Meek
  • Richard O C Oreffo
  • Bo Su
  • Matthew J Dalby
  • Karl E V Burgess
چکیده

Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.

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عنوان ژورنال:
  • Journal of the Royal Society, Interface

دوره 9 73  شماره 

صفحات  -

تاریخ انتشار 2012